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端粒重复序列扩增聚合酶链反应检测肺刷落细胞端粒酶活性 被引量:7

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摘要 用端粒酶聚合酶链反应(PCR)酶联免疫吸附测定(ELISA)法,联合聚丙烯酰胺凝胶电泳(PAGE)和银染色法,检测肺刷落细胞中端粒酶活性,并探讨了其在肺癌诊断中的价值。一、方法TRAPPCRELISA测定具体操作如下:将肺刷落细胞加裂解液10~5...
出处 《中华医学检验杂志》 CSCD 1999年第3期174-174,共1页
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