圆弧青霉脂肪酶基因序列的生物信息学分析

Bioinformatics Analysis on the Sequence of Lipase Gene from Penicillium cyclopium

从圆弧青霉PG37中克隆了碱性脂肪酶(Lip I)基因,并采用生物信息学分析了PG37 LipI基因携带的遗传信息。序列分析结果表明:Lip I DNA序列全长1 480 bp,不存在重复序列,含有5个内含子,5'端存在TATAbox和多个转录因子结合位点;cDNA序列全长1 128 bp,包含了转录起始位点、5'和3'非编码区及858 bp的开放阅读框架;Lip I编码区对TGC、GAC、TTC、CAC、AAG、AAC和TAC这7种密码子使用频率最高;比对LipI基因与Pichia pastoris基因组中的密码子使用频率,其中有21个密码子使用频率的比值相差较大。

The gene encoding alkaline lipase（Lip I） is cloned from Penicillium cyclopium PG37 and the genetic information is analyzed by bioinformatic tool.Sequence analysis showed that Lip I DNA is 1480 bp long with no repeat sequence and five introns,and there is a TATA box and several transcription factor binding sites in the 5＇ end.Lip I cDNA is 1128 bp long with an open reading frame containing 858 bp.It also has transcription start site,5＇ and 3＇ non-coding region.TGC,GAC,TTC,CAC,AAG,AAC and TAC codons are used at highest frequency in Lip I gene.Comparing codon usage frequency of the Lip I with Pichia pastoris genome＇ showed that the ratios of 21 codon usage frequency are different widely.