脱细胞羊膜与小肠黏膜下层对大鼠皮肤缺损修复中血管形成的作用

Function of Acellular Amniotic Membrane and Small Intestinal Submucosa Promotes Vascularization in Vascular Formation for Skin Repairing in Rat

目的:观察脱细胞羊膜(HAM)与小肠黏膜下层(SIS)促进大鼠皮肤缺损修复过程中血管形成的作用。方法:SD大鼠24只,在两侧背部各做1个直径为1.8cm圆形全层皮肤缺损。创面随机分为A组、B组和C组。A组HAM覆盖,B组SIS覆盖,C组纱布覆盖。在2周时处死动物取材,HE染色观察皮肤缺损修复情况。免疫组织化学染色检测VEGF,RT-PCR检测VEGF mRNA的表达。结果:A组、B组愈合较好。C组愈合较差。免疫组织化学染色显示,A、B组VEGF阳性细胞显著多于C组,其中A组最多;RT-PCR结果显示,A、B组比C组表达更多的VEGF mRNA,其中A组最多,差异有统计学意义(P<0.05)。结论:HAM和SIS均能上调皮肤创面组织中VEGF mRNA的表达、增加VEGF的分泌,其中HAM具有更强的促进皮肤缺损修复过程中血管形成作用。

Objective： To investigate repairing effects on vascularization of small intestinal submucosa and acellular amnion for full-thickness skin defects in rats. Methods： Two full-thickness skin defects, which were close to the vertebral column, were created on both sides of the dorsum of SD rats. The skin defects were randomly divided into three groups,including aeellular amniotic membrane in Group A, small intestinal submu- cosa（SfS） in Group B, and physiological saline gauze in Group C（the control group）respectively. After two weeks, the samples were obtained for histological evaluation,immunohistochemical staining of K19 and VEGF, as well as for determination of expression levels of VEGF mRNA with RT-PCR. Results： lmmunohistochemical stain and VEGF showed that there were more positive cells in group A and B rather than in group C,and they are maximum in group A;RT-PCR results showed that the expression levels of VEGF mRNA were higher in group A and B rather than that of group C,and maximum in group A too. The results of the statistical analysis of the research data showed that there were statistically significant differences between the three groups（P〈 0.05）. Conclusion：The results indicate that HAM and SIS enhance expression levels of VEGF, then promotes skin reparation and vascularization in rats.