大肠埃希菌临床株的耐药性调查

Drug-resistance of Escherichia coli:An Investigation of Its Clinical Isolates

目的探讨基层医院大肠埃希菌(ECO)的耐药现状,为临床医师合理使用抗菌药物提供试验依据。方法大肠埃希菌分离鉴定按《全国临床检验操作规程》进行,用CLSI推荐的K-B法进行药敏试验和评价结果。结果医院临床分离的大肠埃希菌对14种常用抗菌药物表现出不同的耐药性,其中,以氨苄西林耐药率最高,达95.0%,而碳青酶烯类抗菌药物亚胺培南敏感率为100.0%;产超广谱β-内酰胺酶(ESBLs)大肠埃希菌的检出率为33.3%。结论基层医院大肠埃希菌的耐药问题也比较严重,应重视耐药菌的监测与控制工作。

OBJECTIVE To investigate the drug-resistance status in clinical isolates of Escherichia coli（ECO） in grass-roots hospital to provide the evidence for reasonable use of antibiotics.METHODS ECO isolates were isolated and identified according to the National Clinical Laboratory Operation Rules.Drug resistance profile was analyzed by K-B method,as recommended by the CLSI.RESULTS The different drug-resistance to 14 commonly used antibiotics had occurred in clinical isolates of ECO.The resistance rate to ampicillin was the highest,accounted for 95.0%.There was not any strain that was resistant to carbopenems such as imipenem.The detection rate of extended spectrum β-lactamases（ESBLs） producing ECO was 33.3%.CONCLUSION Drug-resistance status of ECO in grass-roots hosptitals is very serious.We should attach importance to monitoring and controlling the spread and prevalence of the drug-resistant bacteria.