摘要
目的了解临床分离的革兰阴性杆菌中产ESBLs的分布、耐药性及ESBLs基因型。方法对医院临床分离的革兰阴性杆菌,采用CLSI标准检测产ESBLs菌株,用PCR对ESBLs基因进行分型。结果 120株革兰阴性杆菌分离出38株产ESBLs菌,阳性率31.7%,其中,第1位是大肠埃希菌(53.8%),其次产酸克雷伯菌(35.3%)、肺炎克雷伯菌(33.3%);药敏结果显示,除对碳青酶烯类抗菌药物敏感外,ESBLs阳性株对多种抗菌药物的耐药率均较高,且明显高于ESBLs阴性株;38株产ESBLs菌基因型为TEM、SHV、CTX-M的分别为20、4、6株,有4株同时携带两种β-内酰胺酶基因的菌株,其他基因型4株。结论调查结果表明医院产ESBLs菌分离率较高,主要以大肠埃希菌、产酸克雷伯菌、肺炎克雷伯菌为主,其基因主要以TEM型、CTX-M型流行为主。
OBJECTIVE To study the distribution characteristics of extended-spectrum beta-lactamases(ESBLs) producing strains and the genotype of ESBLs.METHODS The Gram-negative rods were detected by screening and confirmatory test.The genotype of ESBLs was confirmed by PCR analysis.RESULTS From 120 Gram-negative rods 38 ESBLs producing strains(32%) were isolated,the first three isolates with the highest ESBLs producing rate were Escherichia coli(53.8%) Klebsiella oxytoca(35.3%),and K.pneumoniae(33.3%).PCR products revealed that 20 strains harbored TEM type,4 strains with SHV type and 6 strains with CTX-M using their specific primers.Four strains with two kinds of β-lactamase genes were found from the 38 ESBLs producing isolates.CONCLUSION E.coli,K.oxytoca and K.pneumoniae are the most common bacteria producing ESBLs.The ESBLs producing isolates mainly carry the TEM and CTX-M resistant genes.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2010年第18期2746-2748,共3页
Chinese Journal of Nosocomiology