摘要
[目的]研究荔枝核总黄酮(TFL)与胆管阻塞性肝纤维化大鼠肿瘤坏死因子相关凋亡诱导配体(TRAIL)表达的关系以及TFL抗肝纤维的作用。[方法]雄性SD大鼠60只,随机分为3组:假手术组、模型组及TFL给药组,除假手术组外其他大鼠采用胆总管结扎制备肝纤维化大鼠模型。TFL给药组给予TFL200 mg/(kg.d)灌胃,4周后处死所有大鼠,取血清和肝组织进行检测,酶联免疫吸附法检测大鼠血清中TRAIL水平;放射免疫法检测大鼠血清中透明质酸(HA)、层粘蛋白(LN)及Ⅲ型前胶原(PCⅢ)的水平;采用苏木精-伊红染色和Masson胶原染色观察大鼠肝纤维化程度;采用免疫组织化学法检测肝组织TRAIL表达情况。[结果]同模型组相比,TFL给药组肝组织纤维化程度明显改善;TFL给药组和假手术对照组血清TRAIL HA、LN、PCⅢ均显著低于模型组(P<0.05),且TFL给药组和假手术对照组之间血清TRAIL水平差异无统计学意义(P>0.05);血清TRAIL水平与肝组织中TRAIL表达一致;血清TRAIL水平与血清HA、LN、PCⅢ呈正相关(r=0.97,0.95,0.94,P<0.01)。[结论]TRAIL可促进胆管阻塞性大鼠肝纤维化发生发展,TFL可以改善肝纤维化程度,其机制可能与抑制肝内TRAIL的表达有关。
[Objective]To study the relationship between total flavone of Litchi Chinensis Sonn(TFL)and tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)in rat model of liver fibrosis induced by bile duct occlusion and elucidate the role and mechanism of TFL on liver fibrosis.[Methods]Sixty male SD rats were randomly divided into three goups,sham operation,model and TFL treatment group.The hepatic fibrosis rat model was established by bile duct occlusion.TFL with a dose of 200mg/kg·d was intragastrically administrated to treatment group.Four weeks later,animals were sacrificed.Serum TRAIL levels were measured by enzyme-linked immunosorbent assay(ELISA).Serum levels of Hyaluronic acid(HA),Laminin(LN)and procollagen type Ⅲ(PC Ⅲ)were tested through radioimmunoassay(RIA).Liver tissue slides were stained by routine HE and Masson collagen stain to assess the degree of rat liver fibrosis.Expression of TRAIL in liver tissue was measured by immunohistochemical method called Strept-Avidin-Biotin enzyme Complex(SABC).[Results]Compared with model group,the liver tissue of TFL treatment group showed significant improvement in the degree of liver fibrosis.The serum TRAIL levels in the TFL treatment group and sham operation group were significantly lower than the model group(P〈0.05),and there was no significant difference(P〈0.05)between the former two groups.Serum TRAIL levels correlated with the expression of TRAIL and has a positive correlation significantly with the levels of HA,LN and PC Ⅲ in serum.[Conclusion]TRAIL promoted the development of liver fibrosis induced by bile duct ligation in rats,while TFL could ameliorate the degree of liver fibrosis.The mechanism may be related to inhibiting the expression of TRAIL in the liver tissues.
出处
《中国中西医结合消化杂志》
CAS
2010年第4期223-226,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
关键词
荔枝核总黄酮
肝纤维化
肿瘤坏死因子相关凋亡诱导配体
total flavone of Litchi Chinensis Sonn(TFL)
liver fibrosis
tumor necrosis factor related apoptosis-inducing ligand(TRAIL)