摘要
目的研究旋毛虫成囊前期幼虫抗原(pre-encystedlarvaantigens,PELA)的组分和诊断价值。方法应用免疫印迹对比分析PELA和成囊幼虫抗原(ELA)的组分和它们对人及大民旋毛虫病诊断的敏感性和特异性。结果PELA有3条抗原带(分子量分别为:15、17和129kD)与ELA明显不同;对于感染旋毛虫的大鼠,PELA的首次血清阳性反应出现在感染后第10天,而ELA则在感染后第14天,大鼠抗PELA血清抗体出现较早(P<0.01);对22份旋毛虫病人血清,PELA的阳性率为100%,ELA为72.7%,差异具有显著性(P<0.05);除1例鞭虫病人外,其它寄生虫病入及健康人血清,两种抗原均呈阴性反应,两种抗原的特异性无显著性差异(P>0.05)。结论PELA比ELA对早期旋毛虫病的诊断著有更大的价值。
Aim To study the diagnostic values of the pre-encysted larvae (PELA) of Trichinella sPiralis. MethodThe sensitivity and specificity of PELA in diagnoses of rat and human trichinosis were studied by Western blot while the anti-gens of the encysted larvae (ELA) were used as controla- Results There were apparent differences between the antigenic con-stituents of the PELA and ELA1 the PELA first apperred in positive reaction with sera from rats infected with T. sPiralis onthe 10th day after infection, while the ELA on the l4th day. The positive reaction of PELA appeared earlier than that of ELA(P<0. 01). The antibody positive rate of 22 patients with trichinosis was lOO% whi1e the PELA antigens were used. The pos-itive rate was only 72. 2 while the ELA antigens used, the difference was significant (P<O. o5). With sera from 16 cases withascariasis, 9 cases with ancylostomiasis, 9 cases with cysticercosis and l8 normal persons, both the PELA and the ELA yieldednegative reactions. With one of the sera from 8 cases with trichuriasis, however, both of them yielded cross-reactions. Thesensitivity of the PELA was same as that of the ELA. Concluslon The above results demonstrated that PELA was of higherworth than ELA for the early diagnosis of trichinosis.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1999年第3期21-23,共3页
Chinese Journal of Zoonoses
基金
河南省卫生厅资助
河南医科大学基础医学院资助
关键词
旋毛虫
免疫印迹
成囊前期幼虫抗原
Trichinella spiralis
Immunoblot
Pre-encysted larva antigens