摘要
目的为探索日本血吸虫（中国大陆株）22．6kDa抗原（Sj22．6）编码基因用作核酸疫苗的可行性。方法以PCR法对此编码基因改造后将其亚克隆入真核表达载体质粒PCMV－β并转化入大肠杆菌JM109进行大量扩增。将提纯的PCMV／Sj22．6基因重组质粒在体外转化C2C12真核细胞。结果免疫组化试验证明，该重组质粒能够在体外培养的C2C12细胞中表达Sj22.6抗原。结论表明该重组质粒有用作真核疫苗的可能性。
Aim To investigate if the gene fragment of 22. 6kDa antigen of Schistosoma jaPonicum(Sj22. 6)could beexpressed in eukaryotic cells. Metbod It was reformed by PCR to introduce Not I sites at both ends,a Kozak sequence at the5' end and remove stop coden TAA ahead of the first start coden ATG,and then subcloned into a high level eukaryotic expres-sion plasmid pCMV-5 to conruct a naked DNA vaccine pCMV/Sj22' 6. The pCMV/Sj22. 6 was prepared in a large quantityby being transformed into E. coli JM1o9 and then purified and transformed into C2C12 myoblast cells. ResultS Immunohis-tochemical analysis showed that Sj22. 6 protein could be expressed in C2Cl2 cells. Concluslon pCMV/Sj22. 6 recombinantplasmid may be used for a naked DNA vaccine.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1999年第3期48-50,共3页
Chinese Journal of Zoonoses
基金
总理预备金所设专项基金!94-Y-23
