花叶开唇兰的初级培养及再生途径

Primary Culture and Regeneration Ways of Anoectochilus roxburghii

结果表明：培养基的组成对初级培养成活率影响不大，死亡率高、萌发率低是由于花叶开唇兰肉质茎脆弱，抗消毒剂能力差。要提高初级培养成活率应尽量不破坏叶鞘，控制好消毒强度，采种时间以１月为宜。芽的再生途径：一是节间腋芽萌发形成主芽，增殖系数２～２．５；二是原球茎或丛生芽，增殖系数３～４。前一种再生途径形成的芽对外界气温适应能力较强，后一种适应性较差，２８℃以上气温，生长受抑制甚至出现大量死亡现象。试验材料为广西那坡野生花叶开唇兰，植株６节高、茎径大于０．６ｃｍ。

Experiments showed that the ingredients of medium had a little effect on the survival rate; the high mortality and low sprouting rate were due to the weak pulped stem and low resistant to sterilizing.For raising its survival rate, leaf sheeth should be well kepted on the stem and the sterilizing intensity should be in a good control , collecting seedlings in January. The plants are regenerated from both the axillary buds with a multiplication coefficient 2～2 5,and the protocorm with a multiplication coefficient 3～4.The buds from the former way had a good adaption to the outside temperature, while those from the latter way are poor in adaptation and may die at above 28℃.The trial materials were wild Anoectochilus roxburghii collected from Napo county of Guangxi, and plants were 6 nodes high with stem greater than 0 6 cm in diameter.