社区获得性肺炎外周血NF-κB的表达及意义

Expression and significance of NF-κB in patients with community acquired pneumonia

目的研究社区获得性肺炎(community acquired pneumonia,CAP)患者血液中核转录因子(nuclear factor of kappa B,NF-κB)的表达及其意义。方法采用荧光定量检测33例CAP血液中及16例正常对照者血液中NF-κB mRNA的定量表达。结果 CAP患者血液中NF-κB mRNA表达明显高于健康对照者的表达(t=4.48,P=0.0002);重症患者组血液中NF-κB mRNA的定量表达明显高于中度患者组的表达(t=4.22,P=0.0002),中度患者组血液中NF-κB mRNA的定量表达明显轻度患者组的表达(t=2.52,P=0.0205)。结论 NF-κB的过度表达与CAP发生、发展密切相关。

Objective To investigate the expression and significance of nuclear factor of kappa B（NF-κB ）in patients with community acquired pneumonia （CAP）. Methods Thirty-three patients with CAP and 16 health controls were sequentially enrolled in this study.The production of NF-κB mRNA was measured by fluorescent quantitative polymerase chain reaction. Results Expression of NF-κB mRNA in CAP was significantly higher than that in health control（t= 4.48,P= 0.0002）.Expression of NF-κB mRNA in heavy degree CAP group was significantly higher than that of median and mild degree CAP group（t=4.22,P=0.0002）.Expression of NF-κB mRNA in median degree CAP group was significantly higher than that of mild degree CAP group（t=2.52,P=0.0205）.Conclusions Our results suggest expression of NF-κB plays an important role in the development of patients with CAP.