摘要
本研究采用一种新的方法提取核酸，提取的病毒ＲＮＡ纯度高，增加了结果的稳定性；而且操作简单，整个过程只需要１０ｍｉｎ。反转录（ＲＴ）与聚合酶链式反应（ＰＣＲ）在一个反应管中一步操作即可完成，减少了污染机会，提高了反应敏感性和特异性。关键词：聚合酶链式反...
A new approach in preparing viral RNA was used in this study. This approach extracted highly pure viral RNA which increased the reliability of the results, and it was so simple to operate that the pure viral RNA could be obtained within 10minutes. The one-step single-tube RT-PCR was applied in this study, which was easy to operate, less likely to be contaminated, and had high degree of sensitivity and specificity.
出处
《中国卫生检验杂志》
CAS
1999年第4期243-245,共3页
Chinese Journal of Health Laboratory Technology
基金
国家自然科学基金
