丹参愈伤组织细胞固定化及其转化产物的特征

IMMOBILIZATION OF CALLUS TISSUE CELLS OF SALVIA MILTIORRHIZA AND THE CHARACTERISTICS OF ITS PRODUCTS

本文用海藻胶包埋丹参愈伤组织细胞,在LS+KT0.1+NAA1培养液中常温振荡培养一个月左右,培养液抽提物经TLC和HPLC检测,表明该系统可连续分泌丹参的主要成分——丹参酮Ⅱ A和隐丹参酮。此外对丹参愈伤组织细胞悬浮培养、海藻胶包埋条件,同定化细胞的稳定性以及分泌产物的特征等方面进行了比较。

The callus tissue cells of S. miltiorrhiza were immobilized with 3% alginate. Then immobilized cells were cultured in LS+KT 0.1+NAA 1.0+3% sucrose at 25℃. After 27 days or so, the cultures free from cells were extracted with ether and the residues were analyzed by TLC and HPLC. The results showed that immobilized cells could secret some main components of S. miltiorrhiza——tanshinone and cryptotanshinone, especially the same as the roots extract of S. miltiorrhiza. Meanwhile, the immobilized cells stored at 4℃ more than 4 months still preserved its bioactivity to produce the secondary metabolites of S.miltorrhiza.