苯醌修饰葡萄糖氧化酶电极

BENZOQUINO MODIFIED GLUCOSE OXIDASE ENZYME ELECTRODE

本文用苯醌作为电子传递介体,石墨电极为基础电极。葡萄糖氧化酶和苯醌吸附在石墨电极表面,再用戊二醛交联固定。制成的酶电极以电流法测定底物葡萄糖浓度,其线性响应范围为(0—15mmol/L)。本工作测定了介体改良酶电极对葡萄糖的响应值,酶电极的pH范围,介体的循环伏安图谱,以及温度对酶电极的影响。

Much of research on glucose sensor and somc other biosensors have concerned developing systems in which electrons are transferred from the enzyme to some of kind of electrode directel, thereby eliminating the peroxide intermediate step. It is possible to affect this process by direct electrochemistr at chemicall(?)modified electrodes or using chemical mediators in solution or retained on or within the electrode. This paper describes an amp(?)rometric enzyme electrode for the anal sis of glucose. The glucose sensor utilised the mediator benzoquinone to facilitate electron transfer from glucose oxidase to a carbon graphite electrode. A linear response to the glucose concentration over a range (0—15 mmol/L) was observed. Results concerning the electrodes response to glucose, its pH profile, c clic voltammetry of the mediator and the effect of t(?)mperature are presented.