冠心病伴抑郁发作血瘀证/痰浊证的相关基因多态性研究

STUDY ON CORRELATIVE GENE POLYMORPHISM AND BLOOD STASIS/PHLEGM STAGNATION SYNDROME OF CORONARY HEART DISEASE WITH DEPRESSIVE EPISODE

目的:探讨冠心病伴抑郁发作血瘀证和痰浊证与5-HTTLPR、GNβ3C825T、α-ADDUCINGly460Trp基因多态性的关系。方法:选择冠心病伴抑郁发作患者血瘀证115例,痰浊证120例,健康对照者148例,运用多聚酶链式反应技术(PCR)以及限制性内切酶方法检测5-HTT启动子区(5-HTTLPR),GNβ3C825T及ADDUCING460W3种基因多态性,并分析各基因型和等位基因在不同组中的分布频率,并与健康人对照组比较。结果:痰浊证组GNβ3825T等位基因频率与健康人对照组比较分布差异有显著性(χ2=4.568,P=0.033);血瘀证组ADDUCIN460WW基因型和W等位基因分布频率均明显高于健康人对照组,差异有显著性(χ2=12.29,P=0.002;χ2=13.159,P=0.001)。结论:GNβ3C825T可能与冠心病伴抑郁发作痰浊证的遗传机制相关,ADDUCING460W可能是冠心病伴抑郁发作血瘀证的易感基因。

Objective :To explore the relationship between the promoter region of the serotonin transporter gene(5-HTTLPR),G-protein beta3 subunit(GNB3 C825T)and α-dducin gene Gly460Trp(ADDUCIN G460W)polymorphisms and blood stasis/phlegm stagnation syndromes of coronary heart disease with depressive episode.Methods:Coronary heart disease with depressive episode were selected,115 cases were blood stasis syndrome,120 cases were phlegm stagnation syndrome,148 healthy persons were taken as control.The genotypes were determined by polymerase chain reaction(PCR)and allele-specific restriction enzyme analysis,the genotype and allele-frequency were analyzed and compared between the two syndromes with health control.Results:The frequency of GNB3 825T allele in phlegm stagnation syndrome group was obviously higher than that of healthy control,the frequency of ADDUCIN 460W W genotype and W-allele in blood stasis syndrome group was significantly higher than that in healthy control.Conclusion:GNB3 825T maybe related to the phlegm stagnation syndrome of coronary heart disease with depressive episode,ADDUCIN 460W maybe the susceptible gene of the blood stasis syndrome of heart disease with depressive episode.