摘要
在转瓶和２Ｌ搅拌反应器中，利用重组杆状病毒ＡｃＮＰＶ感染ｓｆ９昆虫细胞表达尿激酶原。在转瓶中，细胞接毒密度１２×１０６／ｍＬ、ＭＯＩ＝３０时，尿激酶原活性达到１０６５ＩＵ／ｍＬ。研究了尿激酶原表达过程中葡萄糖、乳酸的代谢变化。实验结果表明细胞状态对尿激酶原的表达水平有显著影响。
The
expression of recombinant protein prourokinase (ProUK) with baculovirus expression vector
system was studied in sf9 insect cell suspension culture using spinner flask and bioreactor.The
expression level 1065IU/mL of ProUK was achieved in spinner flask when MOI=30 and the
infecting cell density 1 2×10 6cells/mL.The metabolism of glucose and lactate was
researched also.The good cell state was very important for maximizing expressionof ProUK.
出处
《生物工程学报》
CAS
CSCD
北大核心
1999年第3期373-377,共5页
Chinese Journal of Biotechnology
基金
国家自然科学基金
