电泳亲和色谱技术分离蛋白质

Separation of Proteins by Electrophoretic Affinity Chromatography

亲和色谱利用亲和配体与目标组分间的特异性结合作用实现对目标组分的纯化，该分离方法分辨率高，在生物物质的分析和分离领域得到日益广泛的应用［１］。亲和色谱在分离过程每一步操作中，液相主体中的溶质分子必须经过一系列扩散过程才能进入到固定相颗粒孔内完成吸附或...

A new kind of electrophoretic affinity chromatography(EAC) was proposed.Separation by EAC was conducted in a multicompartment electrolyzer in which the affinity gel media was packed in one of the central compartments.The presence of an electric field results in the enhancement of transportation of proteins inside the gel metrix during the adsorption and desorption process.This leads to the increase of the speed of separation.The present study was focused on the effects of the electric current on the adsorption and desorption processes.Separation of HSA by EAC with Blue Sepharose 6 Fast Flow TM was successfully achieved,in which 30.24 mg HSA adsorbed on Blue Sepharose was electrophoretically desorpted within 2 hours.This is much higher than the results reported in literature.