摘要
利用PCR技术分别以亲本杉木(Cunninghamialanceolata(Lamb.)Hook.)、柳杉(CryptomeriafortuneiHooibrenk)和杉木×柳杉杂种的总DNA为模板,扩增了叶绿体trnLtrnF和线粒体CoxⅢ基因片段,比较了这些扩增片段的限制性内切酶AluⅠ,DdeⅠ,HinfⅠ,MseⅠ和RsaⅠ的酶切片段多态性,结果表明:F1代的叶绿体DNA为母系遗传,而线粒体DNA为父系遗传。杉木线粒体DNA父系遗传方式与杉科其他植物一致,而叶绿体DNA母系遗传则为在松柏类植物中首次发现。
The inheritance of mitochondrial (mt) DNA and chloroplast (cp) DNA was investigated in intergeneric hybrids from crossing between Cunninghamia lanceolata (Lamb.) Hook. and Cryptomeria fortunei Hooibrenk. The chloroplast trnL trnF region and one intra genic segment of the mitochondrial gene, Cox Ⅲ, were amplified from those of the parents and hybrids by PCR using gene specific primers. Cp and mtDNA polymorphisms of the amplified regions were detected between the parents after restriction digestions. Restriction fragment length polymorphism (RFLP) analysis revealed that all the F 1 individuals possessed Cox Ⅲ restriction fragment patterns (characteristic of the paternal parent Cryptomeria fortunei ) and the trnL trnF region (identical to the maternal parent Cunninghamia lanceolata ) showing that a different mode of inheritance for organelle DNA has occurred in the hybrids. Furthermore, the maternal inheritance of chloroplast DNA is reported here for the first time in coniferophyta.
关键词
杉木
柳杉
叶绿体
DNA遗传
线粒体
Cunninghamia lanceolata , Cryptomeria fortunei , Inheritance of chloroplast DNA, Inheritance of mitochondrial DNA, Restriction fragment length polymorphism