广州市1起诺如病毒急性胃肠炎暴发的实验室检测

Epidemiological Study of a Diarrhea Outbreak Caused by Norovirus in Guangzhou

目的确定1起学校急性胃肠炎暴发原因。方法采集病例粪便标本,直饮水标本,采用实时荧光定量(Real-time)PCR和RT-PCR方法检测病毒核酸,阳性标本测序分析。结果 5份粪便标本中3份呈诺如病毒阳性,2份直饮水标本均呈诺如病毒阳性。3份病例粪便标本病毒株核苷酸序列一致,均为GⅡ-4型,提示病毒同源。结论对诺如病毒进行核酸检测,证实该疫情为一起诺如病毒GⅡ-4型引起的急性胃肠炎暴发。

Objective To analyze the epidemiological characteristics of a Norovirus caused gastroenteritis outbreak in a school in Guangzhou,and provide scientific evidences for the prevention and control of Norovirus gastroenteritis outbreak. Methods Stool and water samples were collected to detect Norovirus by real-time PCR and RT-PCR. Sequence analysiswas conducted on the positive samples. Results Three of 5 stool samples and 2 water samples were positive to the virus. Results The result showed that the sequences of the virus detectedfrom the stool samples of the patients all belonged to the GⅡ-4 species. Conclusion This gastroenteritis outbreak was caused by Norovirus GⅡ-4. Real-time PCR and RT-PCR could provide accurate and reliable results in the detection of Norovirus RNA.