棉铃虫18S核糖体RNA基因的序列分析及其分子系统学

SEQUENCE OF THE 18S RIBOSOMAL RNA GENE OF COTTON BOLLWORM ( HELICOVERPA ARMIGERA ) AND MOLECULAR SYSTEMATICS ANALYSIS

克隆并分析了鳞翅目棉铃虫 Ｈｅｌｉｃｏｖｅｒｐａ ａｒ ｍｉｇｅｒａ （ Ｈüｂｎｅｒ） １８ Ｓ 核糖体 Ｒ Ｎ Ａ 基因 （１８ Ｓｒ Ｄ Ｎ Ａ） 的全序列， 将该序列与鞘翅目、膜翅目、同翅目、双翅目、捻翅目和弹尾目各一种昆虫的同源保守区进行了比较。序列分析结果显示： 鳞翅目和双翅目昆虫在１８ Ｓｒ Ｄ Ｎ Ａ 结构上彼此较为相似， 捻翅目昆虫的１８ Ｓｒ Ｄ Ｎ Ａ 分子结构表现出与其它目昆虫有较大的差异， 但相对与弹尾目昆虫的１８ Ｓｒ Ｄ Ｎ Ａ 较为接近。该结果支持了有关捻翅目属于一个独立的目级分类阶元的论点。

The entire sequence of the 18S ribosomal RNA gene (18S rDNA) of the cotton bollworm ( Helicoverpa armigera ) has been cloned and sequenced. As compared with conservative homologous sequences of other six insects from different Orders, it is found that the 18S rDNA of the cotton bollworm (Lepidoptera) is structurally more similar to that of the fruitfly Drosophila melanogaster (Diptera), suggesting a closer phylogenetic relationship between the two Orders. Phylogenetic relationship analysis reveals the Strepsiptera is closer to Collembola,and indicates being an independent ancient branch in phylogeny.