1984-2002年重庆市59株O_1群霍乱临床分离株耐药性及分子分型研究

Study on Antibiotic Resistance and Molecular Subtyping of 59 Vibrio Cholerae O_1 Clinical Isolates in Chongqing,1984-2002

目的分析重庆市1984-2002年O1群霍乱临床分离株的耐药性和菌株的相关性。方法对59株O1群霍乱临床分离株(小川型20株,稻叶型39株)采用K-B法检测对16种抗菌药物的敏感性,用NotI酶切基因组DNA,经脉冲场凝胶电泳(PFGE)获得电泳图谱,利用BioNumerics软件对图谱进行聚类分析。结果重庆地区O1群霍乱弧菌对复方新诺明耐药、痢特灵和链霉素的耐药严重,耐药率分别为28.81%(17/59)、61.02%(36/59)和30.51%(18/59),人群分离株对丁胺卡那、庆大霉素、妥布霉素、氨苄西林、新霉素和强力霉素敏感,未发现耐药菌株。21株小川型和40株稻叶型O1群霍乱弧菌被分为13种PFGE型18个亚型,从患者分离到的59株O1群霍乱弧菌的相似性值约在88%～100%。结论未发现重庆市O1群霍乱弧菌人群分离株的耐药性有明显改变,小川型和稻叶型的耐药性不同。重庆市O1群霍乱弧菌人群分离株同源性较高,可能为来自同一来源的流行菌株。

Objective To study the antibiotic resistance and the genetic relationships between different Vibrio cholerae O1clinical isolates in Chongqing from 1984 to 2002.Methods The Susceptibility of 59 isolates（20 Inaba strains,39 Ogawa strains）to 16 antimicrobial agents was tested by K-B method,and the chromosoma1 DNA of all Vibrio cholerae O1 isolates were digested in seakem gold agrose with restriction enzyme Not I and plugs were then analyzed by pulsed-field gelelectrophoresis.The pulsed-field gelelectrophoresis（PFGE） patterns of Vibrio cholerae O1 isolates were clustered using BioNumerics software. Results The drug resistance rate to Sulphamethoxazole/trimethoprim,Furazolidone and Streptomycin was 28.81%（17/59）,61.02%（36/59） and 30.51%（18/59）. All the clinical strains which has 88%-100% similarity showed sensitivity to Amikacin,Gentamicin,Tobramycin,Ampicillin,Neomycin and Doxycycline hydrochloride,and 13 PFGE molecular subtyping patterns and 18 molecular subtyping were identified in these strains.Conclusion No change of drug resistance was found in Vibrio cholerae O1 clinical strains in Chongqing form 1984 to 2005,and the antibiotic resistance was different between the serotype Inaba strains and Ogawa strains. The high homology was found in these Vibrio cholerae O1 strains which might come from a popular strain in Chongqing.