抗溴氰菊酯家蝇在不同用药方式下的敏感性变化及其机制

CHANGES OF SUSCEPTIBILITY OF RESISTANT HOUSEFLY TO PESTICIDES AND IT'S MECHANISMS UNDER DIFFERENT INSECTICIDE APPLICATIONS

以具有极高抗水平的抗溴氰菊酯家蝇 Ｍｕｓｃａ ｄｏ ｍｅｓｔｉｃａ ｖｉｃｉｎａ Ｍａｃｑｕａｒｔ Ｄ Ｒ０ 品系为试虫， 模拟田间几种常见的用药方式 （ 混用、轮用、使用增效剂） ， 在室内进行平行汰选， 并以不用药和继续用原药汰选的为比较， 研究试虫在这几种用药方式下的敏感性变化及其变化机制。抗性家蝇用辛溴混剂、辛硫磷以及溴氰菊酯＋ Ｓ Ｖ１ 汰选后， 在 Ｆ１６ （ Ｆ１７） 代以前， 对溴氰菊酯及汰选药剂的抗性发展相对都比较缓慢； Ｆ１６ （ Ｆ１７） 代以后， 用溴氰菊酯＋ Ｓ Ｖ１ 汰选的家蝇对溴氰菊酯的敏感性迅速下降， 抗性发展很快。家蝇对溴氰菊酯的敏感性变化与药剂中溴氰菊酯的选择压有关。生化分析结果表明， 在不同用药方式汰选下， 家蝇体内酯酶、多功能氧化酶、谷胱甘肽 Ｓ转移酶、乙酰胆碱酯酶的酶活或特性发生了不同的变化。

A housefly ( Musca domestica vicina Macquart) strain(DR0) possessing high level resistance to deltamethrin was selected under different insecticide applications in the laboratory, involving alternating insecticide(phoxim), using insecticide mixture(1∶100 mixture of deltamethrin and phoxim) and deltamethrin adding synergist(SV 1). Before generation F 16 (F 17 ),the development of resistance to deltamethrin and selecting insecticides was relatively slow in all three strains; but after that, the rate of development increased, especially that of the deltamethrin+ SV 1 selected strain to deltamethrin.The changes of susceptibility to deltamethrin were correlated with the selection pressure. Biochemical studies showed that,the changes of enzyme activities or properties of esterase, carboxylesterase, mixed function oxidase, glutathione S transferase and acetylcholinesterase were responsible for the changes of susceptibility in the resistant housefly.