组蛋白与转录因子在hAMFR基因启动子序列上的结合及相互作用

Binding and Interaction of Histones and Transcription Factors on the Promoter of hAMFR Gene

采用从鸡红细胞中分离纯化的组蛋白H1、核心组蛋白H2A＋H2B和H3＋H4，以及从HeLa细胞中萃取的含有RNA聚合酶Ⅱ和多种Ⅱ类基因转录因子的可溶性HeLa细胞核抽提物，通过凝胶迟滞电泳，对组蛋白和HeLa细胞核抽提物中的转录因子在人自泌移动因子受体（Humanautocrinemotilityfactor，简称为hAMFR）基因上游启动子序列的相互作用关系进行了初步研究，得到以下结论：组蛋白Hl对hAMFR基因启动子序列的结合是相对稳定的，H1在稳定染色质小体中起着重要的作用。组蛋白及其组份和转录因子可以同时结合在hAMFR基因启动子序列上形成三元复合物，提示转录因子和组蛋白在与DNA竞争结合过程中不是简单地排斥对方。

In this study, histone H1, core histones H2A-H2B and H3-H4 were purified from chicken erythrocytes by hydroxylapatile chromatography. The nuclear extract was prepared from HeLa cells. We investigated the binding and interachon of histones and transcription factors on the upstream sequence of human autocrine motility factor receptor (hAMFR) gene by gel shift mobility assay. We found that the binding of H1 on the promoter sequence of hAMFR gene was relatively stable. We propose that H1 plays an important role in stablizing chromatosome. We also found that histones and HeLa cell extract could form a ternary complex with the DNA template.