δ-生育三烯酚对人结肠癌SW620细胞抑制作用

Anti-proliferative effect of δ-tocotrienol on colorectal cancer cells

目的探讨δ-生育三烯酚对人结肠癌SW620细胞增殖抑制作用。方法采用细胞生长曲线和real-time RT-PCR方法,观察各剂量下δ-生育三烯酚对细胞增殖抑制及对丝/苏氨酸磷酸激酶(GSK-3β)、结肠癌腺瘤性息肉蛋白(APC)及轴蛋白(Axin)在mRNA水平上表达的影响。结果δ-生育三烯酚对人结肠癌SW620细胞有明显抑制作用,24 h细胞生存率为14.4%～88.8%;GSK-3β及APC mRNA表达水平无明显变化,Axin mRNA表达水平为2.24～2.58,明显高于对照组(P<0.05)。结论δ-生育三烯酚能够明显抑制SW620细胞的生长,其机制可能与上调Axin mRNA水平有关。

Objective To investigate inhibitory effect of δ-tocotrienol on proliferation of colorectal cancer（CRC） SW620 cells.Methods SW620 cells were treated with different concentration of δ-tocotrienol（0,20,30,40,60 μmol/L）.Methyl thiazolyl tetrazolium（MTT） method was adopted for the detection of cell growth and real-time reverse transcription polymerase chain reaction（RT-PCR） for the detection of correlated mRNA expression.The contents of GSK-3β,APC,and Axin mRNA were detected.Results δ-tocotrienol inhibited proliferation of CRC SW620 cells.After treated with δ-tocotrienol for 24 hr,the survival rate of the cells was decreased by 14.4% to 88.8%.The RT-PCR study showed that δ-tocotrienol promoted the expression of Axin mRNA（F=6.598,P〈0.05）,but not the GSK-3β mRNA and APC mRNA（P〉0.05）.Conclusion δ-tocotrienol can inhibit SW620 cells proliferation.Its inhibitory mechanism correlates possibly with the adjusting of the associated expression of Axin mRNA.