氮离子注入对耐辐射异常球菌SOD活性的影响及其对Mn-SOD的诱导

THE EFFECT OF N + IMPLANTATION ON SOD ACTIVITY OF DEINOCOCCUS RADIODURANS AND INDUCTION OF Mn SOD

研究了氮离子注入对耐辐射异常球菌（Ｄｅｉｎｏｃｏｃｃｕｓｒａｄｉｏｄｕｒａｎｓ）细胞内超氧化物歧化酶（ＳＯＤ）活性的影响及其对ＭｎＳＯＤ的诱导。结果表明，当２０ｋｅＶ氮离子的注入剂量低于８×１０１４ｉｏｎｓ／ｃｍ２时，Ｄ．ｒａｄｉｏｄｕｒａｎｓ中ＳＯＤ活性变化不大，当剂量在８×１０１４～６０×１０１４ｉｏｎｓ／ｃｍ２范围内时，ＳＯＤ的活性随着注入剂量的增大逐渐提高，但大于６０×１０１４ｉｏｎｓ／ｃｍ２时，则逐渐下降；加入对不同金属辅基的ＳＯＤ同工酶活性抑制剂Ｈ２Ｏ２和氯仿乙醇的研究表明，中高剂量下氮离子注入诱导的是Ｄ．ｒａｄｉｏｄｕｒａｎｓ中ＭｎＳＯＤ活性的提高，在正常生理条件及小于８×１０１４ｉｏｎｓ／ｃｍ２的剂量下，Ｄ．

The induction of Mn SOD and effect of N + of 20ke V implantation on SOD activity of D.radiodurans were investigated. The results were as follows: (1) SOD activity of D.radiodurans had a little change when implantation dose was less than 8×10 14 N +/cm 2, and increased gradually from 8×10 14 to 60×10 14 N +/cm 2, and then decreased rapidly; (2) the test of adding H 2O 2 and CHCl 3 CH 3CH 2OH which could inhibit different types of SOD showed that most SOD activity of D.radiodurans was constituted by Fe SOD in general physiology and less then 8×10 14 N +/cm 2 dose, and higher implantation dose induced increase of Mn SOD activity.