自絮凝酵母颗粒连续发酵生产酒精的新工艺

INDUSTRIAL SCALE UP OF CONTINUOUS ETHANOL FERMENTATION USIN G YEAST FLOCS

用既有优良酒精发酵性能，又具有强自絮凝能力的融合酵母株ＳＰＳＣ，在单釜有效容积为１０ｍ３的四釜并联气升环流悬浮床生物反应器系统中，进行了连续发酵生产酒精的研究。以玉米为原料，双酶法制糖，过滤得到清糖液作为底物，在稀释速率为０１／ｈ的条件下，终点发酵液中酒精浓度为７０～８０ｇ／Ｌ，残余还原糖和残余总糖分别为２～３ｇ／Ｌ和３～５ｇ／Ｌ，悬浮床反应器的设备生产强度达到７～８ｇ（ＥｔＯＨ）／（Ｌ·ｈ）。

Inducing yeast cells to self flocculate as a cell immobilization method, an industrial scale up plant composed of 4 air lift suspended bed bioreactors in parallel and with a total volume of 400m 3 was established and operated. During a more than 6 months continuous operation, the effluent contained ethanol of 70～80g/L and residual sugar less than 5g/L and the ethanol productivity of 7～8g/L·h -1 was achieved.