不同采收期北柴胡指标性成分的动态积累研究

Study on Dynamic Accumulation of Index Components from Bupleurum chinense in Various Collecting Periods

目的:以河南嵩县地区北柴胡为样本,研究柴胡皂苷a、d,挥发油及醇溶性浸出物的动态变化规律,探讨北柴胡的最适采收期。方法:以柴胡皂苷a、d的含量,柴胡挥发油的吸光度以及柴胡醇溶性浸出物为指标,采用高效液相-蒸发光散射检测器(HPLC-ELSD)及紫外分光光度法为分析方法。结果:不同采收期北柴胡中的柴胡皂苷a、d,挥发油及醇溶性浸出物均存在明显的差异。其中柴胡挥发油的含量10月份最高;柴胡皂苷a的含量9月份最高;柴胡皂苷d的含量12月份最高;而醇溶性浸出物10月份最高。结论:确定了河南地区北柴胡的最适采收期,为柴胡药材的采收及生产提供了一定的科学依据。

Objective： To study the dynamic change law of volatile oil,saikosaponin a,d and alcohol-extract from Bupleurum chinense at Songxian region in Henan province,and to explore the optimal harvest period of Bupleurum chinense.Methods： With the contents of saikosaponin a and d,absorbance of volatile oil and percentage of alcohol-extract as indexes,HPLC-ELSD and ultraviolet spectrophotometry were successively used to analyze them.Results： There are obvious differences among the contents of volatile oil,saikosaponin a,d and alcohol-extract in various collecting periods sample,the absorption of volatile oil in distillation was the highest in October,the content of saikosaponin a was the highest in September,the saikosaponin d in December and the percentage of alcohol-extract in October.Conclusion： The optimal harvest period of Bupleurum chinense at Songxian region in Henan is identified,which can provide scientific basis for crude drug production and processing.