二色波罗蜜根皮总黄酮提取纯化工艺研究

Studies on Technology Optimization for Extraction and Purification of Total Flavones from Root Bark of Artocarpus styracifolius

目的:研究二色波罗蜜根皮总黄酮的最佳提取、纯化工艺条件。方法:采用正交试验设计,以总黄酮含量为考察指标研究二色波罗蜜根皮总黄酮的最佳提取工艺条件;以总黄酮的静态吸附量和解吸率为考察指标筛选大孔吸附树脂,并优化总黄酮的纯化工艺条件。结果:最优提取工艺条件为乙醇浓度为60%,浸提温度为50℃,浸提次数为1次,料液比为1∶8。HPD-500型大孔吸附树脂对二色波罗蜜根皮总黄酮有较好的吸附分离性能,以22.0mg总黄酮/g树脂的上柱量配制成50.0 mg/mL的上柱液,上HPD-500大孔吸附树脂柱,静置1 h,用4 BV的水洗脱杂质,再用4 BV的80%乙醇洗脱,得到的精制品中总黄酮含量为86.4%,树脂富集倍数为5.33。结论:采用以上提取、纯化条件进行二色波罗蜜根皮总黄酮的制备,简便、实用,可以为工业生产提供参考。

Objective：To study the technology optimization for extraction and purification of total flavones from root bark of Artocarpus styracifolius.Methods：The optimum extraction conditions were investigated by the contents of the total flavones,using orthogonal test;Static adsorption capacity and desorption rate were employed as examine items for the screening of optimum macroporous resin and optimum technology for the purification of total flavones with selected macroporous were also investigated.Results：The optimum extraction conditions were as follows：using 60% alcohol of seven times than amounts of original material soaking 12 hours,extracting once with hot reflux method at 50 ℃.HPD-500 type macroporous resin showed better adsorption and desorption property.The optimum purification conditions were as follows：the sample solution was prepared at the concentration of 50.0 mg/mL,subjected to HPD-500 type macroporous resin column chromatography with a load ratio of 22.0 mg total flavones per gram of resin.After standing for 1 hour,the column was eluted with 4 BV water before being eluted with 4 BV 80% alcohol.The purity of the product was 86.4%,which enhanced the content of total flavones by 533%.Conclusion：The optimum conditions for extraction and purification of total flavones from root bark of Artocarpus styractifolius are convenient and practical,and could be used as a reference for industrial production.