L_(7811)鼠腹水肿瘤细胞^(31)P核磁共振的研究

THE STUDIES OF CELL FROM L_(7811) LEUKEMIA MOUSE BY ^(31)P-NMR

用^(31)P核磁共振技术(^(31)P-NMR)研究了L_(7811)鼠腹水肿瘤细胞和615系鼠胸腺细胞(正常对照细胞)。结果发现在肿瘤晚期阶段,L_(7811)腹水肿瘤细胞的含磷化合物未进入完全不活跃状态。此外,腹水肿瘤细胞的磷脂组成与含量亦有明显改变。因此,^(31)P-NMR谱可做为观察肿瘤细胞内能量生成和某些磷脂合成宏观动态过程的一项参考指标。

Using ^(31)P-NMR to study the high energy phosphates of mouse L_(7811) ascites cells with thymic ceils from 615 line mouse as control. The results showed there was clear differences between them.Even in the late stage of growth, L_(7811) ascites cells was not completely in an inactive state. For example, the ratio of phosphocreatine (PCr) and α-, β-, γ- adenosine triphosphate (α-, β-, γ-ATP) to phosphate (Pi) was greatly decreased but not completely inactive. The ratio of sugar phosphate (Sp) to phosphocholine (Pcho) plus phosphoethanolamine (PEtn) increased, while glycero (3) phosphocholine (GroPCho) plus glycero(3) phosphoelhano-lamine (GroPEtn) decreased.Preliminary studies showed that ^(31)P-NMR spectra are sensitive monitor to the progressive changes of the high energy phosphate metabolism of the L_(7811) ascites growth.