猪繁殖与呼吸综合征病毒流行株Nsp2基因的克隆及序列分析

Cloning and Sequence Analysis of Nsp2 Genes of Porcine Reproductive and Respiratory Syndrome Virus Prevalence Strains

采用RT-PCR方法,从吉林省部分地区猪场的病料中扩增猪繁殖与呼吸综合征病毒(PRRSV)的Nsp2基因并测序。应用DNAStar 7.0、ClustalX1.83、MEGA4.0软件对测序结果进行分析,并与NCBI上已登录的PRRSV代表毒株的Nsp2基因进行序列比对,结果显示,得到的Nsp2基因与VR-2332、CH-1a等代表毒株的一致性为62.0%～87.5%,与国内高致病性PRRSV毒株JXA1、HUB2等的一致性为97.8%～98.9%;氨基酸序列比对结果显示与VR-2332、CH-1a等代表毒株的一致性为34.4%～59.0%,与国内高致病性PRRSV毒株JXA1、HUB2等的一致性为95.1%～98.4%。因此,引起吉林省部分地区猪场发生猪繁殖与呼吸综合征的PRRSV与国内流行的高致病性PRRSV毒株亲缘关系较近。

The nonstructural protein 2（Nsp2） genes of porcine reproductive and respiratory syndrome virus（PRRSV） were cloned using RT-PCR from porcine tissue samples in some pig farms in Jilin province.The sequences of Nsp2 were sequenced and analyzed using DNAStar 7.0,ClustalX 1.83 and MEGA 4.Homology searches were performed by means of BLAST programs by NCBI.Identities of nuclear acid sequences and amino acid sequences deduced from Nsp2 genes cloned were 62.0% to 87.5% and 34.4% to 59.0% with PRRSV standard strains VR-2332 and CH-1a and so on,were 97.8% to 98.9% and 95.1% to 98.4% with Chinese highly pathogenic PRRSV strains JXA1 and HUB2 and so on.Therefore,PRRSVs leading to the infectious disease in some pig farms in Jilin province were high homology with highly pathogenic PRRSV strains.