猪伪狂犬病病毒受体nectin-2基因的克隆与分子特征

Molecular Cloning and Characteristics of cDNA Encoding Porcine nectin-2 for PRV Receptor

为了进一步了解猪nectin-2基因的结构与功能,本研究采用生物信息学结合RT-PCR的方法从猪脑组织中克隆到了猪nectin-2基因,并对其核苷酸序列和推导的氨基酸序列进行了比较分析。猪nectin-2基因的编码区含有1440个核苷酸,编码479个氨基酸,其中信号肽由32个氨基酸组成,胞外域由330个氨基酸组成,含有2个潜在的N-糖基化位点和6个半胱氨酸残基,跨膜区由23个氨基酸组成,胞浆区由94个氨基酸组成,猪nectin-2基因与犬、马、家鼠、人、恒河猴、牛、黑猩猩的nectin-2基因核苷酸序列同源性分别为85.4%、85.7%、78.6%、82.1%、82.1%、81.9%和82.1%;推导氨基酸序列的同源性分别为84.5%、83.0%、74.7%、75.7%、76.4%、78.4%和75.5%。本试验为进一步深入研究猪伪狂犬病病毒与宿主之间的关系奠定了基础。

In order to understand the structure and function of porcine nectin-2 gene,we cloned and sequenced the cDNA encoding porcine nectin-2 from the brain of pig.The 1440 bp cDNA of porcine nectin-2 encoded a polypeptide of 479 amino acids consisting of a 32-residue putative signal peptide,a 330-residue ectodomain,a 23-residue transmembrane domain,and a 94-residue cytoplasmic domain.The ectodomain contained 2 potential N-linked glycosylation sites and 6 cysteine residues.The nucleotide sequence similarity of nectin-2 between pig and dog,horse,house mouse,human,rhesus monkey,cattle,Chimpanzee were 85.4%,85.7%,78.6%,82.1%,82.1%,81.9%,82.1%,and the amino acid sequence similarity were 84.5%,83.0%,74.7%,75.7%,76.4%,78.4%,75.5% respectively.This study will lay a foundation for understanding the interactions of PRV with host.