京海黄鸡IGFBP-3基因外显子1和内含子1部分序列多态性及其与生长繁殖性状的相关性

Genetic Diversity of IGFBP-3 Gene Exon 1 and Partial Sequence of Intron 1 and their Association with Growth and Reproductive Performance in Jinghai Yellow Chicken

以京海黄鸡母鸡为材料,采用PCR-SSCP技术对IGFBP-3基因外显子1及内含子1部分序列多态位点进行研究,并计算基因型频率、基因频率、卡方值和部分遗传多态性指标。结果表明,在外显子1上没有检测到多态位点,在内含子1上检测到1个多态位点,该位点为中度多态,在160 bp处发生T到G的突变,导致BB、AB型的56日龄体重显著大于AA型(P<0.05),AA型的11月产蛋数显著大于BB、AB型(P<0.05)。由此初步推断,内含子1对产蛋及生长发育很可能有一定的促进作用,A为产蛋的优势基因,B为体重的优势基因。

To Jinghai yellow chicken hens as material,using PCR-SSCP technique to study IGFBP-3 gene exon 1 and partial sequence of intron 1 polymorphic site and calculate the genotype,gene frequency,chi-square value and some indicators of genetic polymorphism.The results of exon 1 was not detected polymorphic site,in intron 1 of the partial sequence detected in more than one polymorphic site,the locus polymorphism as a moderate,in the first 160 bp took place T to G mutation,leading to BB and AB types 56 days of age,body weight was significantly greater than AA types（P〈0.05）,AA type egg in November was significantly greater than the number of BB and AB types（P〈0.05）.From this initial inference of intron 1 on the egg-laying and growth was likely to have a certain role in promoting,A gene for the advantage of egg-laying,B gene for the advantage of weight.