摘要
PH-30是参与精卵质膜间相互作用中最有特征性的候选分子之一。为了检测PH-30基因在白绒山羊睾丸中特异表达情况,试验提取公山羊睾丸组织总RNA,反转录合成cDNA,并根据GenBank中公布的3种动物PH-30 cDNA序列的保守区设计1对引物,PCR扩增后纯化回收目的片段,连接pGM-T载体,挑取阳性克隆进行测序,经序列分析鉴定目的片段。结果表明,PCR扩增获得448 bp目的片段,与绵羊PH-30同源性99%,与牛PH-30的同源性为92%。多组织的RT-PCR研究结果表明,该基因只有在白绒山羊睾丸组织中特异性表达,而在其附睾头、体、尾部组织中没有表达。
PH-30 is the best characterized molecule involved in the sperm-egg plasma membrane interaction.In this study,total RNA was prepared from cashmere goat testis and different parts of epididymis,cDNA fragment was amplified by RT-PCR using specific primers,then PCR product was cloned into pGM-T Easy vector.The nucleotide was sequenced and analyzed,99% identity with sheep PH-30 and 92% homology with bovine PH-30 were found.Expression analysis by RT-PCR revealed that PH-30 was only expressed in the testis when compared to caput,corpus,cauda epididymis.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第9期135-138,共4页
China Animal Husbandry & Veterinary Medicine
基金
中国博士后基金项目(57546)
国家自然科学基金项目(3056010330740043)
