应用实时荧光定量PCR测定牛体细胞端粒长度

Bovine Somatic Cell Telomere Length Measurement by Quantitative PCR

本研究为了验证应用实时荧光定量聚合酶链反应(quantitative PCR,Q-PCR)测定牛基因组端粒长度的可行性,选取18个不同来源牛耳成纤维细胞抽提基因组DNA为样本,对Q-PCR和经典的Southern印迹法进行了相关性分析。结果显示,Q-PCR测定端粒长度相对T/S为1.16±0.24,Southern印迹法测量端粒平均TRF值为16.99 kb±0.85 kb,两种方法获得的结果相关性分析R2=0.5612(P<0.01),因此实时荧光定量PCR是一种测定牛基因组端粒长度的可靠的方法。

In order to test the possibility of quantitative PCR（Q-PCR） in the measurement of bovine telomere length,we chose 18 different DNA samples of bovine ear fibroblast,and then analyzed the relativity of quantitative PCR and Southern blotting methods.The results showed that the relative T/S ratio of Q-PCR was 1.16±0.24,the mean TRF of Southern blotting was 16.99 kb±0.85 kb.The analysis of the relativity of two methods showed that R2= 0.5612（P〈0.01）,so we got the conclusion that Q-PCR was a reliable method in the measurement of bovine telomere length.