pH梯度对平板膜中单体菌紫质分子光电响应的影响

THE EFFECT OF pH GRADIENT ON PHOTOELECTRIC RESPONSE OF MONOMERIC bR BLM

利用重组技术把单体bR包入DMPC脂质囊泡中,通过吸收光谱和圆二色谱的测定,证明bR在囊泡中仍以单体存在。同时测定了外界pH梯度对单体bR脂质囊泡BLM光电响应的影响,观察到pH值当内池大于外池其差值超过2时就可产生光电响应信号极性的反转,并对此结果作了一定的讨论。

Monomeric bR was reconstituted into DMPC vesicles. Absorption spectrum and CD spectrum show bR molecules in DMPC vesicles were in monomeric state. We have measured the effect of pH gradient on photoelectric response of monomeric bR BLM. When the pH in inside container was higher than the pH in outside container and the difference of pH was more than 2, we have observed the change of polarity from positive to negative. Also we have discussed the reason of the change of polarity of photoelectric response.