膀胱移行细胞癌rasP21和P53表达的临床意义

Clinical significance of rasP21 and P53 oncoprotein expression in transitional cell carcinomas of bladder

目的探讨ｒａｓＰ２１和Ｐ５３表达与膀胱移行细胞癌病理分级、临床分期、复发、肿瘤大小及患者年龄的关系。方法采用免疫流式细胞光度术与病理学相结合的方法对４７例膀胱移行细胞癌细胞ｒａｓＰ２１和Ｐ５３蛋白表达进行检测分析。结果ｒａｓＰ２１和Ｐ５３阳性表达率随病理分级升高而渐升高（Ｐ＜０．０５）。Ｔ２～Ｔ４期ｒａｓＰ２１、Ｐ５３的阳性表达率高于Ｔ１期（Ｐ＜０．０５）。ｒａｓＰ２１阳性者复发率为５１．６％（１６／３１），阴性者１２．５％（２／１６），Ｐ５３阳性者复发率为４５．７％（１６／３５），阴性者１６．６％（２／１２）。ｒａｓＰ２１、Ｐ５３的阳性表达率与肿瘤大小及患者的年龄无统计学差异。结论ｒａｓＰ２１和Ｐ５３的表达可反映膀胱移行细胞癌的恶性程度和预后。

Objective To elucidate rasP21 and P53 expression in bladder transitional carcinoma and their relationship with pathological grade,clinical stage,recurrence,tumor size and patients′ ages.Methods rasP21 and P53 expression at protein level were studied in 47 cases of bladder transitional carcinoma with flow cytometric(FCM)and pathological methods.Results With the differentiation of the bladder cancer,rasP21 and P53 positive expressions were significantly increased.There were statistical significance on grade Ⅰand Ⅱ as compared with grade Ⅲ ( P < 0.05 ),rasP21 and P53 positive expressions were higher in stage T2 to T4 as compared with T1( P < 0.05 ).Recurrence rate of rasP21 and P53 positive cases was 51.6% (16/31)and 45.7% (16/35),respectively,while recurrence rate of rasP21 and P53 negative cases was 12.5% (2/16) and 16.6% (2/12),respectively.rasP21 and P53 positive expression had no statistical significance with tumor size and patients′ ages.Conclusion rasP21 and P53 expression may be a valuable marker in evaluation of malignant degree of bladder transitional carcinoma.