体外治疗性超声辅助尿激酶溶栓的实验研究

Study on external therapeutic ultrasound augments urokinase induced thrombolysis in vivo

目的评价体外治疗性超声（ＥＴＵＳ）辅助尿激酶（ＵＫ）溶栓的效果和安全性。方法选用日本大耳白兔，共２４只建成双侧股动脉血栓模型。随机分组接受不同方法（对照、ＥＴＵＳ、ＵＫ、ＥＴＵＳ＋ＵＫ）溶栓，超声频率０．８ＭＨｚ、声强１．２Ｗ／ｃｍ２、占空比１００％。ＵＫ分１５０００／ｋｇ与３００００μ／ｋｇ两种剂量，经耳缘静脉于３０ｍｉｎ内给入。结果单用ＥＴＵＳ组无明显溶栓，血管未出现再通。ＥＴＵＳ＋ＵＫ组与对应的ＵＫ组比较，溶栓量增加（Ｐ＜０．０１），血管再通率提高（Ｐ＜０．０５），血管再通所需时间缩短（Ｐ＜０．０１）。组织学检查显示ＥＴＵＳ应用组血管结构无损伤。结论０．８ＭＨｚ、１．

Objective To assess the efficacy and safety of external therapy ultrasound on enhancing thrombolysis with urokinase in a rabbit femoral artery thrombosis model.Methods Blood clots were produced in both femoral artery segments of 24 rabbits and randomized to different groups (neither ultrasound nor urokinase,ultrasound alone,urokinase alone,ultrasound plus urokinase).Urokinase was infused via the marginal ear vein.Continuous wave ultrasound at a frequency of 0.8 MHz and intensity of 1.2 W/cm 2 was applied through water over the area of occlusion.Results Neither clot lysis nor recanalization occurred in ultrasound alone group.In camparison with the urokinase alone group,the ultrasound plus urokinase group caused a significant enhancement of both clot lysis ( P < 0.01 ) and rate of recanalization ( P < 0.05 ),and shorter time of recanalization( P < 0.01 ).Vessel damage was not detected histologically in rabbits exposed to ultrasound.Conclusion Continuous wave ultrasound at 0.8 MHz, 1.2 W/cm 2 can be used safely to accelerate urokinase induced thrombolysis in vivo.