增殖细胞核抗原及P53在舌癌中的表达

The expression and clinical significance of proliferating cell nuclear antigen ( PCNA) and P53 in tongue cancer

目的：研究ＰＣＮＡ、Ｐ５３在舌癌中表达的相关性及与肿瘤病理分级、淋巴结转移、预后的关系。方法：应用免疫组化ＳＰ法，观察４６例病理确诊的舌癌石蜡标本中ＰＣＮＡ指数及Ｐ５３阳性率的表达。结果：ＰＣＮＡ在所有病例均有不同程度表达，４６例中２８例呈Ｐ５３阳性表达（６０．９％）。Ｐ５３阳性组中ＰＣＮＡ指数明显高于Ｐ５３阴性组（Ｐ＜０．００１）。ＰＣＮＡ指数随舌癌病理分级的升高而增加（Ｐ＜０．０２５）。Ｐ５３阳性率在Ⅱ、Ⅲ级舌癌明显高于Ⅰ级舌癌（Ｐ＜０．０５）。在有淋巴结转移及术后生存小于３年组中ＰＣＮＡ指数及Ｐ５３阳性率均明显高于无淋巴结转移及术后生存３年以上组（Ｐ＜０．００１，Ｐ＜０．０５）。结论：联合检测ＰＣＮＡ及Ｐ５３对判断舌癌恶性度，预测淋巴结转移趋势和预后及指导临床治疗有重要价值。

Objective:To study the relationship between PCNA and P53 expressions and relation between PCNA,P53 and pathological grade,lymphnode metastasis,prognosis respectively in tongue cancer.Methods:Using SP immunohistochemical method,the PCNA indices and positive rate of P53 were examined in 46 paraffin embedded specimens of tongue cancer diagnosed by pathology.Results:PCNA expression was found in all the cases with an average index(%) of 42.3±22.11 (9.1～87.2).P53 expression was found in 28 out the 46 cases (60.9%).Heigher PCNA index and P53 positive rate were observed in the cases with heigher pathological grade,with lymphnode metastasis,and in the cases who died within 3 years after surgical treatment than those with lower pathological grade,without lymphnode metastasis,and in those who were alive 3 years after operation ( P <0.05).Heigher PCNA index was also found in P53 positive cases than in the negative ( P <0.001).Conclusion:Examination of PCNA and P53 is valuable in determining the degree of malignancy,predicting lymphnode metastasis and prognosis of the tumor as well as in directing clinical treatment.