中国南北地区两个人群HLA-A座位DNA分型的比较研究

A comparative study of HLA A locus in northern and southern Chinese by means of PCR/SSOP typing

目的对我国北京和广州的各一个群体进行ＨＬＡ－Ａ座位分型并进行比较研究。方法用ＰＣＲ／ＳＳＯＰ的ＤＮＡ基因分型方法，使用引物１对，探针５４种。用同位素磷３２标记探针进行ＤＮＡ杂交，用Ｘ片曝光判断结果。结果共检出ＨＬＡ－Ａ基因１８种，南北人群有一定程度的差异，表现在北方北京地区人群中Ａ＊０２０５、０２１０和Ａ＊２９０１未检出，Ａ＊２６０１、３００１和３１０１等基因高于南方广州人群；而南方广州群体中Ａ＊３１０１、３２０１和６８０１未检出，Ａ＊０２０３、１１０１高于北方群体。并发现血清型Ａ２有６种基因亚型，包括Ａ＊０２０１、０２０３、０２０５、０２０６、０２０７、０２１０，其中以０２０１为主体。结论中国南北人群的遗传背景存在一定的差异，Ａ２基因亚型分型在器官移植配型中有重要意义。

Objective To compare the HLA A locus in a popolation selected from Beijing(northern group)with that in a population selected from Guangzhou (southern group). Methods HLA typing was performed by using PCR/SSOP method. A pair of primer and 54 probes were used. Results In 18 alleles identified, the differences in gene frequency between northern and southern Chinese were found. In northern group , A*0205,0210 and 2901 were absent, and the frequencies of A*2601, 3001 and 3101 were higher than those of southern group; while in southern group, A*3103, 3201 and 6801 were absent, the frequencies of A*0203 and 1101 were higher than those of northern group; and in addition, six subtypes of A2 serological specificity, namely A*0201, 0203, 0205, 0206, 0207 and 0210, were found, in which A*0201 predominated.Conclusion There are some differences in the genetic background of northern and southern Chinese. A2 subtypes have important implications for unrelated donor transplantation.