性别及年龄对外周血淋巴细胞中21号染色体分离的影响

Influence of aging and sex on chromosome 21 segregation in human lymphocytes

目的阐明性别与年龄对体细胞中２１号染色体分离的影响。方法用细胞松弛素Ｂ处理不同年龄及性别正常人外周血淋巴细胞获得的双核细胞，以２１号染色体近着丝粒特异性探针进行荧光原位杂交，同时检测２１号染色体丢失及不分离。结果具有４个信号点的双核细胞数与年龄的相关系数为－０．３５（Ｐ＜０．０１），２及６个信号点的双核细胞数与年龄的相关系数分别为０．１８和０．３８（Ｐ＜０．０１），２１号染色体不分离数和微核细胞数与年龄的相关系数分别为０．５６（Ｐ＜０．０１）和０．７０（Ｐ＜０．０１）。结论在不同年龄正常人外周血淋巴细胞中，微核数随年龄的增加而增加。２１号染色体不分离与年龄的关系极显著，无论在体内还是体外，２１号染色体不分离远远高于丢失。

Objective To determine the influence of aging and sex on the segregation of chromosome 21 . Methods Lymphocytes were obtained from healthy donors of different ages, both sexes, and were treated with cytochalasin B. Binucleated cells were hybridized with chromosome 21 specific probe, and simultaneously, chromosome 21 loss and nondisjunction were detected. Results The coefficients of correlation between age and binucleated cells containing 4,2 and 6 signals were -0.35( P< 0 01 ), 0.18 and 0.38( P<0.01) respectively. The coefficients of correlation between age and nondisjunction of chromosome 21 and micronuclei were 0.56( P<0.01) and 0.70( P<0.01) respectively. Conclusion There is a significant increase with age in the number of micronuclei and missegregation. Nondisjunction of chromosome 21 is much more frequent than loss in vivo and in vitro. No significant difference in nondisjunction between male and female was noted. Age effect is more significant in female than in male.