呼吸道合胞病毒感染对哮喘患儿单个核细胞表达白细胞介素4和5的影响

Mite induced interleukin 5 and interleukin 4 expressions in peripheral blood mononuclear cells from asthmatic children with respiratory syncytial virus infection

目的观察呼吸道合胞病毒（ＲＳＶ）感染对哮喘患儿螨诱导单个核细胞（ＰＢＭＣ）表达白细胞介素４（ＩＬ４）和ＩＬ５的影响及其与哮喘的关系。方法用酶联免疫吸附试验（ＥＬＩＳＡ）及逆转录（ＲＴ）聚合酶链反应（ＰＣＲ）法分析了３０例螨过敏哮喘患儿［其中ＲＳＶ感染者１６例（Ａ组），无ＲＳＶ感染者１４例（Ｂ组）］和１０名年龄匹配的正常对照组（Ｃ组）的外周血ＰＢＭＣ经螨诱导表达ＩＬ４及ＩＬ５的水平，并分析其与血清嗜酸细胞阳离子蛋白（ＥＣＰ）浓度及哮喘症状的关系。结果（１）Ｂ组螨诱导ＰＢＭＣＩＬ４［（３４±２４）ｎｇ／Ｌ］和ＩＬ５的分泌结果和ＩＬ５［（２６±１５）ｎｇ／Ｌ］的分泌与Ｃ组的ＩＬ４［（８±５）ｎｇ／Ｌ］的分泌比较，差异有显著性（Ｐ均＜０．０１），同时螨诱导ＩＬ５的分泌结果和ＩＬ５［（６±５）ｎｇ／Ｌ］呈剂量依赖关系。Ａ组的ＰＢＭＣ经螨刺激后ＩＬ５的释放水平［（６８±４３）ｎｇ／Ｌ］与Ｂ组比较，差异有显著性（Ｐ＜０．０５）。两组ＩＬ５ｍＲＮＡ表达量与ＩＬ５蛋白水平的变化相似。（２）Ａ组患者的血清ＥＣＰ水平［（７３±４７）μｇ／Ｌ］与Ｃ组［（１８±１２）μｇ／Ｌ］比较，差异有显著性（Ｐ＜０．?

Objective To investigate the effects of respiratory syncytial virus(RSV) infection on mite induced interleukin 5 and interleukin 4 expressions in cultures of PBMC from asthmatic children and their correlation with asthmatic symptoms. Methods IL 5 and IL 4 expressions in mite (D. Pteronyssinus) stimulated PBMC were assessed ex vivo by ELISA and reverse transcription polymerase chain reaction (RT PCR) in 16 allergic asthmatic children with RSV infection(A) and 14 patients without RSV infection(B), as well as 10 normal controls(C) (5.1± 2.4 yrs). Meanwhile their correlation with serum ECP or current wheezing was analysed in patients after RSV infection. Results (1) Stimulation of PBMC with mite antigens induced significantly higher IL 4 [(34±24) ng/L vs (8±5) ng/L, ( P <0.01)] and IL 5 [(26±15) ng/L vs (6±5) ng/L] release in allergic patients(B) as compared with healthy controls(C). The release was in a mite dose dependent manner. Meanwhile, IL 5 level in group A (A) was the highest (73±47) ng/L, P <0.05 compared with (B)). Similar results were obtained with IL 5 mRNA expressions. (2)Serum ECP level was significantly higher in group A [(73±47) μg/L] than in group C [(18±12) μg/L, P <0.01]. There was a significant correlation between IL 5 release and ECP levels ( r =0.49, P <0.05). (3) Mite induced IL 5 release in PBMC was significantly increased in the group with wheezing symptom [(71±40) ng/L compared with the asymptomatic group (34±29) ng/L, ( P <0.05)]. Conclusions Our findings indicated that during RSV infection, mite induced IL 5 expression in PBMC of asthmatic children was up regulated, which may enhance activation of eosinophils and precipitate or exacerbate asthma symptoms.