正交法优选连翘酯苷提取工艺

Optimization of Extracting Technology for Forsythiaside by Orthogonal Test

目的:通过正交试验,优选连翘叶中连翘酯苷的最佳提取工艺。方法:采用正交设计的方法,考察加水量、提取时间、提取次数和提取温度对连翘酯苷得量的影响,优选出最佳提取条件。结果:各因素的影响依次为:C>A>B>D,提取次数(C)为主要因素,加水量(A)、提取时间(B)、提取温度(D)为次要因素。提取的最佳工艺条件为用16倍的水,60℃温浸法提取3次,每次提取1 h。结论:优选得到的连翘酯苷的提取工艺简单、稳定、可行。

Objective：To establish the optimal extraction technology of forsythiaside by orthogonal test.Method：The amount of water,extraction time,extraction times and extraction temperature were set as index for orthogonal test.Result：The factors were：C A B D.Extraction times（C） was as the main factor and the amount of water（A）,extraction time（B）,extraction temperature（D） as a secondary factor.The optimum conditions for extraction was as follows：16 times of water,soaking extraction 3 times at 60 ℃,each time extracting for 1 hour.Conclusion：The optimum extraction process for forsythiaside was simple,stable and feasible.