20种中药水溶性糖GC/MS指纹图谱与寒热药性的Fisher判别分析

GC/MS Fingerprints and Fisher Discrimination Analysis on Water-soluble Sugar from 20 Chinese Herbs with Cold and Heat Properties

目的:探讨中药寒热药性与水溶性糖的相关性,进一步阐明中药四性与物质基础的关系。方法:选取10种寒性中药和10种热性中药,提取水溶性糖,分别加入盐酸羟胺、吡啶和乙酸酐进行乙酰化反应,测定水溶性糖的GC/MS指纹图谱,利用Fisher方法建立判别函数,分析实验结果。结果:Fisher判别函数实现了对20种中药寒热药性的准确识别,组内回代一致率为100%。结论:Fisher可以作为判别中药寒热药性与水溶性糖关系的统计学工具,水溶性糖是中药寒热药性的物质基础之一。

Objecive：To discuss the relationship between water-soluble sugar and cold-heat properties and further reveal correlations of four properties and material basis.Method：Water-soluble sugars were purified from 10 herbs with cold properties and 10 herbs with heat properties.After acetylation,GC /MS fingerprints of water-soluble sugars were determined and the results were analyzed by Fisher.Result：Discriminant function had been established by Fisher.The Fisher discriminant analysis could distinguish the cold and heat properties between 20 herbs that the back substitution in group was 100%.There was difference between the drugs with cold and heat properties in the GC /MS fingerprints,which corresponded to the different water-soluble sugars in the cold and heat herbs.Conclusion：The Fisher analysis could differentiate the herbs with cold and heat properties,and it was an effective statistical tool to study the relationship between the cold and heat properties by water-soluble sugar.It concludes that the water-soluble sugar is one of the material basis of four properties of herbs.