野生白苏子中挥发油的研究

Study on Essential Oil Obtained from Seed of Herbal Perilla frutescens

目的:提取野生白苏子中的挥发油,确定其挥发油中的组分,探讨其生物活性,为开发利用这一资源提供理论依据。方法:用水蒸气蒸馏法从白苏子中提取挥发油,用气相色谱-质谱联用技术对挥发油组分进行分离和结构确定,对白苏子挥发油进行抗氧化试验和抗菌试验。结果:从白苏子挥发油中鉴定出56个组分,占全油的92.77%,白苏子挥发油有明显地抗氧化活性和抗菌活性。结论:白苏子挥发油中主要以单萜和倍半萜为主,含量较高的组分是紫苏酮10.32%,异白苏烯酮9.73%,丁香烯7.69%,α-里哪醇6.32%,瓜菊酮5.28%等。白苏子挥发油对.OH有明显的清除作用,白苏子挥发油对实验菌株均有明显的抑制和灭活作用,特别是对金黄色葡萄球菌CMCC26112株和白色假丝酵母菌CMCC850216株的作用表现得更为显著。

Objective：The essential oil was extracted from the seed of wild Perilla frutescens,and the constituent was analyzed to provide scientific proof for the search of biological activity compositions from P.frutescens to empolder the natural resource.Method：The essential oil from the seed of wild P.frutescens was extracted by steam distillation,and the components of the essential oil were separated and structurally identified by gas chromatography-mass spectrometry.The essential oil was investigated by anti-oxidation activities and antimicrobial activities.Result：Fifty-six components were identified and accounted for 92.77% of the all peak area.The essential oil had anti-oxidation activity and antimicrobial activity.Conclusion：The main chemical constituents of the essential oil were monoterpenoids and sesquiterpenoids compounds.The major components were perillaketone（10.32%）,isoegomaketone（9.73%）,caryophyllene（7.96%）,α-linalool（6.32%）,cinerone（5.28%）.The essential oil had obvious anti-oxidation activities for scavenging of.OH,killed and inhibited the growth of testing bacteria,especially against Staphylococcus aureus CMCC26112 and Candida albicans CMCC850216.