新加下瘀血汤对糖尿病大鼠肾脏骨形成蛋白7和转化生长因子β1表达的影响

Effects of Xinjia Xiayuxue Tang on Bone Morphogenetic Protein 7 and Transforming Growth Factor β1 in Kidney of Diabetic Rats

目的:观察新加下瘀血汤对糖尿病大鼠肾脏组织中转化生长因子β1(TGFβ1)和骨形成蛋白7(BMP7)表达的影响,以探讨新加下瘀血汤对糖尿病大鼠肾脏的保护作用及其机制。方法:SD大鼠随机分为正常对照组、糖尿病模型组和新加下瘀血汤治疗组。以四氧嘧啶复制糖尿病模型。12周后观察空腹血糖、血肌酐水平及24h尿微量蛋白,光镜(PAS染色)观察肾脏病理,RT-PCR技术检测肾脏组织中BMP7,TGFβ1及纤溶酶原激活物抑制因子1(PAI1)mRNA表达。结果:与模型组比较,新加下瘀血汤明显降低糖尿病模型大鼠血糖、肌酐水平和尿蛋白的排泄,肾脏病理显示肾损害明显减轻,BMP7表达明显增加,TGFβ1及PAI1 mRNA表达明显降低。结论:新加下瘀血汤对糖尿病肾脏有明显的保护作用,其机制可能与其降低肾脏TGFβ1表达,增加BMP7的表达进而降低PAI1表达有关。

Objective：To explore the protective mechanism of Xinjia Xiayuxue Tang on kidneys in diabetic rats by investigating the effect of Xinjia Xiayuxue Tang on bone morphogenetic protein 7（BMP7） and transforming growth factor β1（TGFβ1）in kidneys of diabetic rats.Method：SD rats were divided into control group and diabetes model group.The diabetes model was established by injection of alloxan through vena caudalis,and then were randomly divided into diabetes model（DM） group and Xinjia Xiayuxue Tang-treated group.After 12 weeks,The fast-ing blood glucose（FBG）,serum creatinine（Scr）,urine protein（Upro） were checked with biochemical methods.The morphological changes of renal tissue were examined under microscopy on sections stained with PAS.The mRNA expression of BMP7,TGFβ1 and plasminogen activator inhibitor 1（PAI1）were tested by RT-PCR.Result：Compared with DM model group,in Xinjia Xiayuxue Tang-treated group,FBG,Scr and Upro were markedly decreased（P 0.01）;renal damage were significantly reduced,the expression of TGFβ1 and PAI1 mRNA were significantly reduced,BMP7 mRNA expression was greatly increased.Conclusion：Xinjia Xiayuxue Tang exerted protective effect on diabet-ic nephropathy probably via elevation of BMP7 and reduction of TGFβ1,then reducing the expression of PAI1.