加味芎归汤对大鼠局灶性脑缺血损伤的保护作用和血液流变学的影响

Effects of Modified Xionggui Decoction on Protection from Cerebral Ischemia Injury and its Influence on Hemorheological Parameters in Rats

目的:研究加味芎归汤对大鼠局灶性脑缺血损伤的保护作用和血液流变学指标的影响。方法:采用Wistar大鼠,以尼龙线栓右中脑动脉阻塞法制备局灶性脑缺血模型,加味芎归汤(0.38,0.76,1.52 g.kg-1)ig 7 d,另设模型、假手术和阳性药(通脉颗粒)对照,按Bederson法进行神经功能评分,取血进行血液流变学检查,TTC染色法测定脑组织缺血范围,并进行脑组织病理学检查。结果:加味芎归汤和通脉颗粒给药5 d时,神经功能障碍明显改善;给药7 d时,高切全血黏度、全血还原黏度和血浆黏度降低;脑组织缺血范围明显缩小;脑组织病理改变减轻。结论:加味芎归汤对脑组织缺血损伤有明显保护作用,可能与其降低血液黏度有关。

Objective：To study the effects of Modified Xionggui Decoction（MXD） on protection from cere-bral ischemia injury and its influence on hemorheological parameters in rats.Method：Seventy of Wistar rats were undergone right middle cerebral artery occlusion（MCAO） according the method of intraluminal thread,then were randomly divided into 5 groups.Four groups were administered MXD（0.38,0.76,1.52 g.kg-1） and Tongmai Keli（2.7 g.kg-1） respectively.MCAO model group and sham-operation control group of fifteen rats were administered with the same volume of water.The neurologic findings were scored,hemorheological parameters were measured,and the size of cerebral infarction was determined by TTC-stained method,and histopathologic changes were observed.Result：MXD and Tongmai Keli significantly improved neurologic function after administered drugs for 5 days.MXD and Tongmai Keli significantly reduced blood viscosity of high shear rate,reduced blood viscosity and plasma viscos-ity,and significantly lessened the size of cerebral infarction and lightened histopathologic changes after administered for 7 days.Conclusion：MXD may improve the obstruction of neurologic function,and protect brain from ischemia injury.The effect of MXD might be related with improvement in hemorheological parameters.