加味四物汤降压作用及对左心室肥厚影响的研究

Effect of Supplemented Siwu Decoction on Blood Pressure and Left Ventricular Hypertrophy in Spontaneous Hypertension Rats

目的:观察加味四物汤降压及对左心室肥厚的作用。方法:将自发性高血压大鼠(SHR)分为模型组、加味四物汤高、中、低剂量组(31.52,15.74,7.81 g.kg-1)及牛黄降压丸(0.17 g.kg-1),卡托普利组(7.88 g.kg-1)。测定用药后各时段的血压变化,并在用药前及8周后分别观察左心室质量指数(LVMI)、心肌胶原含量及Ⅰ,Ⅲ型胶原比值和心肌组织形态学改变。结果:加味四物汤各剂量可使血压不同程度下降,连续给药8周高剂量组血压显示持续降低,与模型组比较有显著性差异(P<0.01)。各剂量组左室质量指数(LVMI)均减小,高剂量组及卡托普利组与模型组比较存在显著差异(P<0.05)。各药物组Ⅰ、Ⅲ胶原比值不同程度的改善。形态学观察显示,与模型组比较,加味四物汤组的心肌纤维结构与分布较好,断裂、坏死和水样变性较轻,胶原沉着较少,毛细血管腔开放,周围渗出较少。结论:加味四物汤具有一定的降压作用,可能对SHR的左心室肥厚有改善作用。

Objective：To observe the effect of Supplemented Siwu Decoction（SSWD） on blood pressure and left ventricular hypertrophy of spontaneous hypertension rats（SHR）.Method：Sixty SHR were randomly divided into six groups（n = 10,each）：SSWD high dose group（31.52 g.kg-1.d-1）,SSWD middle dose group（15.74 g.kg-1.d-1）,SSWD low dose group（7.81 g.kg-1.d-1）,model group（with normal saline）,Niuhuang Jiangya Wan group（0.17 g.kg-1.d-1） and Captopril group（7.88 g.kg-1.d-1）.SBP and left ventricular mass index（LVMI）,the content of left ventricular myocardical interstitial collagen and the collagen ratio of typeⅠto type Ⅲ at each groups were measured.Result：SSWD of high dose reduced SBP significantly compared with the model group（P 0.01）,the antihypertensive effect was remaining and lasting during whole investigation.LVMI of high dose group reduced remarkly compared to that in the model group（P 0.05）,the content of left ventricular myocardical collagen was lower than that in the model group,the deposition of left ventricular myocardical interstitial collagen of high dose group decreased pronouncedly compared to the model group.The outcomes from the microscope and elec-tronic microscope indicated that the damages in SSWD group were much slighter than those of the model group.Conclusion：SSWD could effectively lower blood pressure and led to regression of left ventricular hypertrophy in SHR.