HPLC测定广西莪术不同炮制品超微细粉中牻牛儿酮的含量

Determination of Germacrone in Superfine Powder of Different Processed Products of Curcuma kwangsiensis by HPLC

目的:研究广西莪术4种不同炮制品超微粉碎前后对其中牻牛儿酮含量的影响。方法:超微细粉的制备采用低温(-30±2)℃球磨技术,磨细至1.0～2.0μm尺寸;采用HPLC,色谱柱大连依利特Hypersil ODS2 C18柱(4.6 mm×250 mm,5μm);流动相乙腈-水(65∶35);紫外检测波长为210 nm。结果:不同炮制品超微细粉中牻牛儿酮的含量在粒径为75～53μm的普通粉末较高,而粒径为1.0～2.0μm的莪术超细粉末含量明显降低。结论:超微细粉的粒径对广西莪术炮制品中牻牛儿酮含量有明显影响,含量并不随粒度的减小而增加,为广西莪术不同炮制品提取工艺提供试验参考数据。

Objective： To determine the content of germacrone from different processed products of Curcuma kwangsiensis before and after the superfine comminution.Method： With the ball milling technology at low temperatures（-30 ± 2） ℃,the size of the superfine powder was between 1.0-2.0 μm.The HPLC analysis was performed on Hypersil ODS2 C18（4.6 mm × 250 mm,5 μm）,the mobile phase was acetonitrile-water（65∶ 35）,the detection wavelength was at 210 nm.Result： The germacrone content of commom powder（75-53 μm） was higher than the content of the superfine powder（1.0-2.0 μm）.Conclusion： The size of superfine powder influenced the content of germacrone of the four different processed products of C.kwangsiensis evidently,and this offered referenced data for the different processed products of C.kwangsiensis.