灵芝配方颗粒中麦角甾醇的含量测定

Determination of Ergosterol in Lingzhi Dispensing Granules

目的:建立灵芝配方颗粒中麦角甾醇的含量测定方法。方法:反相HPLC,流动为甲醇-水(99∶1),检测波长为281 nm,流速1.2 mL·min-1,柱温25℃。结果:麦角甾醇对照品进样量在0.223 7～1.342μg呈良好线性关系,回归方程为Y=1 104.468X+1.168,r=0.999 9,回收率为103.1%,RSD为0.48%(n=6)。结论:本法简便易行,重复性好,可作为灵芝配方颗粒的质量控制方法。

Objective： To establish a quantitative method for determination of ergosterol in Lingzhi Dispensing Granule.Method： A RP-HPLC method was used to determine the content of ergosterol with methanol-water（99∶ 1） as the mobile phase and detection wavelength was 281 nm,The flow rate was 1.2 mL.min-1 and the column temperature was 25 ℃.Result： The linearity of ergosterol were good in range of 0.223 7～1.342 μg,and the regression equation was Y=1 104.468 X ＋ 1.168（r=0.999 9）.The average recovery was 103.1% and the RSD value of repeatability was 0.48%（n=6）.Conclusion： This method is simple and reproducible,and it can be used for quality control of Lingzhi Dispensing Granule.