香青兰中总黄酮和单体的含量测定

To Determine Content of Total Flavonoids and Robinin-7-o-β-D-xylosylglucoside from Dracoephalum mololarie

目的:考察香青兰中单体(刺槐素-7-o-β-D-葡萄糖苷)、总黄酮的含量。方法:采用紫外分光光度法(UV)测定总黄酮含量;采用高效液相色谱法(HPLC)测定单体含量,RP-C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸水(26∶74),流速为1.1 mL·min-1,柱温室温,检测波长为326 nm。结果:单体(刺槐素-7-o-β-D-葡萄糖苷)含量为0.33%,回收率为98.62%;总黄酮含量为1.90%,回收率为97.59%。结论:所建方法准确、简便,为科学地评价香青兰药材的质量提供依据。

Objective： To determine the content of total flavonoids and robinin-7-o-β-D-xylosylglucoside fron Dracoephalum mololarie.Method： The content of total flavonoids was determined by ultraviolet spectrophotometry（UV）.The content of robinin-7-o-β-D-xylosylglucoside was determined by HPLC.The analysis was performed on a RP-C18 column（4.6 mm × 250 mm,5 μm） with aceconitrile-0.1% phosphoric acid（26∶ 74） as mobile phase at a flow rate of 1.1 mL.min-1,and at room temperature.The detection of wave length was set at 326 nm.Result： The content of robinin-7-o-β-D-xylosylglucoside was 0.33%,and recovery rate was 98.62%.The content of total flavonoids was 1.90%,and recovery rate was 97.59%.Conclusion： The method is suitable for the determination of flavonoids components from D.mololariea.