电针郄门穴对室性心动过速模型大鼠心率及血浆一氧化氮含量的影响

Influence of Electro-acupuncture at Ximen (PC4) on the Heart Rate and Plasma Nitric Oxide of the Ventricular Tachycardia Rat Models

目的探讨电针郄门穴对室性心动过速(VT)大鼠心率的作用及其机制。方法将SD大鼠40只随机分为正常组、模型组、电针郄门组、电针尺泽组,每组10只。正常组不做任何处理,其余3组采用股静脉注射氯化铯(CsCl)的方法建立大鼠VT模型,并连续观测心电图,记录心率变化;电针郄门组和电针尺泽组在造模成功后分别电针郄门穴和尺泽穴治疗5min;4组大鼠均采用比色法检测血浆中一氧化氮(NO)含量。结果模型组、电针郄门组及电针尺泽组大鼠在注射CsCl造模后心率均显著增快(P<0.01),模型组与电针尺泽组在整个观察期内心率未见恢复,电针郄门组在电针治疗5min后心率明显减慢,与模型组及电针尺泽组比较差异均有统计学意义(P<0.01)。4组大鼠血浆NO含量比较,模型组较正常组明显降低(P<0.01),而电针郄门组较模型组和电针尺泽组均明显升高(P<0.01或P<0.05),而与正常组比较无统计学差异(P>0.05)。结论电针郄门穴可能通过抑制心脏交感神经,兴奋迷走神经,增加NO的释放,调整心率而抗心律失常。

Objective To observe the effect and mechanism of electro-acupuncture at Ximen （PC4） on the heart rate of the ventric- ular tachycardia model rats. Methods Forty SD rats were randomized into 4 groups with 10 in each： normal group, model group, electro-acupuncture Chize （LU5） group and electro-acupuncture Ximen （PC4） group. The normal group was not treated. The other 3 groups were made ventricular tachycardia （VT） models with cesium chloride （CsCI） injected into femoral vein. The electrocardiogram （ECG） and heart rate of the models were observed and recorded. After modeling, the electro-acupuncture Chize （LUS） group and electro-acupuncture Ximen （PC4） group were treated with electro-acupuncture at Chize （LUS） and Ximen （PC4） respectively for 5 minutes. The colorimetry was adopted to detect the content of and plasma nitric oxide （NO） of all 4 groups. Results After CsCI in- jection, the heart rate of the model group, electro-acupuncture Chize （LU5） group, and electro-acupuncture Ximen （PC4） group was significantly quickened （P〈0.01）. The heart rate of the model group and electro-acupuncture Chize （LU5） group was not recovered in the whole observing period. The heart rate of the electro-acupuncture Ximen （PC4） group was significantly reduced 5 minutes after treatment, compared with the model group and electro-acupuncture Chize （LU5） group, the difference was significant （P〈0. 01）. In comparison of the 4 groups, the content of NO of the model group was significantly decreased than that of the normal group （P〈 0.01 ）, NO of the electro-acupuncture Ximen （PC4） group was significantly increased than that of model group and electro-acupuncture Chize （LUS） group （P〈0.01 or P〈0. 05）, and it was not significantly different from that of the normal group. Conclusion The electro-acupuncture at Ximen （PC4） can regulate the heart rate for anti-arrhythmia through inhibiting the heart sympathetic nerve and exciting the vagus nerve to increase the NO release.